Assessment of the Antimicrobial activity of Punica granatum Extract against common bacterial Isolates from Dongola hospitals. 2023-2024

ABSTRACT:

Recently, natural products have been evaluated as sources of antimicrobial agents with efficacies against a variety of microorganisms, Plants have been in use for thousands of years to treat health diseases. The main aim of this study is that due to increasing concerns about the development of antimicrobial resistance among pathogenic bacteria, so alternative strategies are sought that do not use antibiotics to reduce pathogenic bacteria from foods and patients. Punica granatum has been commonly employed as a crude drug in traditional medicine. The antibacterial activity of methanolic Extract of Punica granatum Peels, Pith, and Seeds were evaluated using Agar Disc diffusion susceptibility in different concentrations (50 mg/ml, 25mg/ml, 12.5mg/ml, 6.25mg/ml) against twelve multi-drug isolates (S.aureus, E.coli, K.pneumoniae, S.paratyphi A, Shigella spp,, P.vulgaris and P.aeruginosa). All clinical isolates were observed to be susceptible to all concentrations of peels extract. Pith extract have antibacterial activity against all isolates except S.praratyphi A. Seeds extract show no activity against isolates except P. vulgaris.

Keywords: Antimicrobial, bacteria, Punica granatum Extract, herbal antimicrobials