IR Spectrum of Oil Extract from Anise (Pimpinella Anisum L.) Dynamics

Abstract

Fourier transform infrared spectroscopy (FTIR) is an effective method for the characterization of essential oils, including anise oil (Pimpinella anisum L.). FTIR analysis revealed the presence of functional groups characteristic of the majority of compounds, especially anethole, responsible for the aromatic and bioactive properties of the oil. The FTIR spectrum of anise oil shows the following main bands: 3400–3200 cm⁻¹ (O–H vibrations due to phenolic compounds), 3000–2850 cm⁻¹ (aliphatic C–H vibrations), 1640–1600 cm⁻¹ (aromatic C=C vibrations), 1500–1450 cm⁻¹ (C–H bending vibrations), 1260–1020 cm⁻¹ (ethereal C–O–C vibrations, characteristic of anethole) and 900–700 cm⁻¹ (C–H out-of-plane bending vibrations of the aromatic ring). These spectral signatures allow the identification of the main chemical composition of the oil and highlight the role of anethole as the dominant bioactive component. FTIR thus proves to be a rapid and non-invasive method for the qualitative analysis of anise oil, contributing to quality control studies and phytochemical research.